植物形成蛋白（formin）负责调控细胞生长、细胞分裂、细胞极性生长等诸多细胞生物学过程，是植物细胞中最为重要的一类蛋白质。拟南芥 formin家族基因众多，有 21 个成员，据 N 端结构域的不同分为 I 型和 II 型 formin，不同 formin 基因间功能存在互补性，单独敲除其中几个 formin 基因不能观察到明显表型变化，这使得研究 formin 成员体内功能十分困难。本论文利用CRISPR/Cas9 编辑技术，对拟南芥中Ⅰ类 formin 基因中的三个进化较近且存在功能冗余的 AtFH4、AtFH7 和 AtFH8 基因进行多基因敲除。已成功完成AtFH4、AtFH7 与 AtFH8 的单基因、双基因及三基因的 CRISPR/Cas9 编辑载体的构建。利用农杆菌介导的浸花法，将携带 CRISPR/Cas9 编辑质粒转化野生型拟南芥。后期转化筛选所得到的转基因材料一定可为进一步研究AtFH4、AtFH7 与 AtFH8 三个基因所编码蛋白的相关功能研究提供重要的实验材料。

Plant formins are proteins responsible for regulating numerous cellular biological processes such as cell growth, cell division, and polar cell growth, making them one of the most important classes of proteins in plant cells. The Arabidopsis formin gene family is numerous, with 21 members, divided into type I and type II formins based on the N-terminal domain. There is functional complementarity between different formin genes; knocking out a few formin genes individually does not result in noticeable phenotypic changes, making it very difficult to study the in vivo functions of formin members. This paper utilizes CRISPR/Cas9 editing technology to knock out multiple genes of three closely related and functionally redundant type I formin genes in Arabidopsis: AtFH4, AtFH7, and AtFH8. The construction of single-gene, double-gene, and triple-gene CRISPR/Cas9 editing vectors for AtFH4, AtFH7, and AtFH8 has been successfully completed. Using Agrobacterium-mediated floral dip method, the CRISPR/Cas9 editing plasmids were transformed into wild-type Arabidopsis.