现今，在大型食肉动物面临诸多威胁，种群数量大幅减少的情况下，中等食肉动物在陆地生态系统的能量流动和物质循环中发挥了越来越重要的作用。珲春东北虎保护区是东北虎豹国家公园的一部分，当地动植物多样性丰富。亚洲狗獾（Meles leucurus）是虎豹公园内中等食肉动物的一种，为杂食性哺乳动物，构成了东北地区食物链食物网的重要一环，对于种子的传播、森林底层物种群落和多样性有重要影响。本研究利用高通量测序和DNA宏条形码技术对亚洲狗獾的食性组成进行了部分分析，初步完成了对狗獾特异性阻抑引物的设计。研究发现：（1）本实验设计的阻抑引物12S-leu4-3c3可基本完全阻抑狗獾DNA的扩增，同时对水獭、赤狐等同地区中小型食肉动物的DNA无明显阻抑效果，是较适宜于狗獾的特异性阻抑引物。（2）亚洲狗獾在春季取食食物种类丰富，至少取食16种动物，具有广食性。其中蚯蚓、小型哺乳类动物是其主要的食物。同时，根据食性检测中发现的鹿属及犬属可认为当地狗獾在春季有食腐行为。

Nowadays, due to the population decline of large carnivores by multiple threats, mesa-carnivores play an increasingly important role in energy flow and matter cycling in terrestrial ecosystems. Northeast Tiger and Leopard National Park, is rich in wildlife and plant diversity. Meles leucurus, an omnivorous mammal, is one of the mesa-carnivores in Northeast Tiger and Leopard National Park. It forms an important part of the food web at temperate forest in Northeast China, and plays an important role in seed dispersal and species community and diversity in forest. In this study, high-throughput sequencing and DNA macrobarcoding were used to partially analyze the diet composition of Asian badgers, and a preliminary design of specific blocking primers for badgers was completed. The results showed that: (1) The inhibition primer 12S-leu4-3c3 designed in this study could almost completely inhibit the DNA amplification of badger, and had no obvious inhibitory effect on the DNA of small and medium-sized carnivores from otter and red fox. Therefore, 12S-LEU4-3C3 is a specific inhibition primer suitable for badger. (2) Asian badgers feed on a wide variety of food in spring, at least 16 kinds of wildlife. Earthworms and small mammals are the main food. In addition, according to the deer and Canis found in the feeding test, it could be considered that local badgers had scavenging behavior in spring.