在谷胱甘肽还原酶（GR）的催化作用下，氧化型谷胱甘肽（GSSG）可以与还原型辅酶Ⅱ（NADPH）发生反应生成还原型谷胱甘肽（GSH）和氧化型辅酶Ⅱ（NADP⁺）。利用GSH对AuNCs的荧光增强效应，实现对GSH的定量检测。此外，为了提高方法的灵敏度，向AuNCs中加入一定量的二氧化锰纳米片（MnO₂NSs）以猝灭其荧光，使MnO₂NSs与GSH的反应作为开启荧光的机制。据此，本论文开展了如下研究：

（1）以GSSG为还原剂和保护剂合成AuNCs，考察以它为荧光探针定量检测GSH的灵敏度和选择性。

（2）在（1）的基础上，将外加的GSH替换为GSSG、GR和NADPH的混合溶液，通过控制变量的方法分别建立GR活性浓度、NADPH浓度与AuNCs的荧光强度变化程度的线性关系，从而实现对GR活性和NADPH的定量检测。

实现了在pH=7.2，温度为37℃的条件下，对GR活性和NADPH含量快速、高选择性的检测，为GR活性和NADPH含量的检测提供了新的方法和思路。

Under the catalysis of glutathione reductase (GR), oxidized glutathione (GSSG) can react with reduced coenzyme Ⅱ (NADPH) to form reduced glutathione (GSH) and oxidized coenzyme (NADP⁺). By using the fluorescence enhancement effect of GSH on AuNCs, and the quantitative detection of GSH could be realized. In addition, in order to improve the sensitivity, manganese dioxide nanosheets (MnO₂NSs) were added to AuNCs to quench the fluorescence, and the reaction between MnO₂NSs and GSH was used as the mechanism to activate the fluorescence. Based on this, this paper carries out the following research:

(1) AuNCs were synthesized using GSSG as reducing agent and protectant, and the sensitivity and selectivity of quantitative detection of GSH with GSSG as a fluorescent probe were investigated.

(2) On the basis of (1), the added GSH was replaced by the mixed solution of GSSG, GR and NADPH, and the linear relationship between the active concentration of GR, the concentration of NADPH and the change degree of fluorescence intensity of AuNCs was established by controlling variables, so as to realize the quantitative detection of the activity of GR and NADPH.

Under the condition of pH=7.2 and temperature 37℃, the GR activity and NADPH content were detected quickly, sensitively and highly selectively, which provided a new method and idea for the detection of GR activity and NADPH content.