野生型酿酒酵母中，Hsp90的同源蛋白Hsp82在应激条件下会发生明显的核富集现象。Hsp82I578F突变体表达的Hsp82在第578位的亮氨酸突变为苯丙氨酸，使得Hsp82在对数期不能进入细胞核。当此突变发生时，酵母的时序型衰老（CLS）加速，但复制型衰老（RLS）的变化还不清楚。同时，酵母细胞衰老也可能和受Hsp82影响的基因组稳定性的丧失有关。 本课题从以下两方面初步探究了Hsp90在酿酒酵母细胞衰老中的功能。 首先，构建Hsp82条件性敲除菌株（因完全敲除致死），通过检测突变率，对比其与Hsp82I578F突变体及野生型三种菌株下的基因组稳定性。初步实验表明，三种菌株的基因组稳定性无明显差异。 其次，利用毒蛋白Barnase-抗毒蛋白Barstar系统改进了传统的母细胞富集法，以对比野生型和Hsp82I578F突变体的RLS。然而，由于菌株构建过程中引入的Barnase点突变导致系统效率降低，因此该方法还未用于正式实验检测中。

In wild-type Saccharomyces cerevisiae, Hsp82, a homologous protein of Hsp90, is observed to have significant nuclear accumulation under some stress conditions. In the mutant Hsp82I578F, the leucine at position 578 of Hsp82 is mutated to phenylalanine, making Hsp82 unable to enter the nucleus in the log phase. When this mutation occurs, it accelerates chronological life span (CLS) of yeast, but the effect on its replicative life span (RLS) is unclear. At the same time, yeast cell aging may also be associated with loss of genomic stability affected by Hsp82. This study focused on the function of Hsp90 in cell aging in Saccharomyces cerevisiae from the following two aspects. First, the Hsp82 conditional knockout strain was constructed (the strain died after Hsp82 completely knockout), and the genomic stability under the Hsp82I578F mutant and wild type strains was compared by detecting their mutation rate. Preliminary experiments showed that there was no significant difference in the genomic stability of the three strains. Secondly, by introducing the Barnase/Barstar system, the traditional method of studying yeast RLS, mother enrichment program (MEP), was improved to compare the RLS of wild-type and Hsp82I578F mutants. However, due to the point mutations of Barnase during the construction of strains, the efficiency of the system has decreased, and it has not been used in formal experiments.