白色念珠菌是一种与人共生的条件致病性真菌，白色念珠菌依据周围所处环境条件呈现不同形态，分别是芽殖酵母态和假菌丝态以及真菌丝态。菌丝形态与白色念珠菌致病性具有紧密的联系。菌丝形成可以通过多种条件进行诱导，如血清、中性PH 7、CO2等。之前的研究证明ESCRT系统的关键蛋白ATPase Vps4能够影响白色念珠菌菌丝萌发和极性维持。 为了进一步阐明ESCRT系统在白色念珠菌菌丝生长过程中的作用，我们通过基因敲除，荧光标记，菌丝诱导等方法对Vps4的辅助因子Vta1进行了研究。初步发现：与Vps4相比，Vta1同样能够影响菌丝萌发和极性维持，并且还能够引起特有的菌丝生长异常现象。初步说明在菌丝生长过程中Vta1不但可以通过影响Vps4来发挥作用，而且还可能通过其他途径参与白色念珠菌菌丝生长过程。 我们还通过GST pull down、酶活力测定方法首次在体外证明了白色念珠菌中Vta1与Vps4的相互作用以及Vta1对Vps4的ATP水解酶活性的增强作用。这表明白色念珠菌Vta1可以作为Vps4的辅助因子在体内进一步增强Vps4的正常功能，这与我们在白色念珠菌体内的研究相一致。

Candida albicans is a conditional pathogenic fungus that symbiotically interacts with humans. Candida albicans present different forms depending on the surrounding conditions, namely yeast and pseudomycelium and true hyphae. Hyphal formation is closely related to the pathogenicity of Candida albicans. Mycelial formation can be induced by a variety of conditions, such as serum, neutral-to-alkaline pH7, CO2 and so on. Previous studies have demonstrated that the key protein Vps4 of the ESCRT system can affect hyphal formation of Candida albicans. Through gene knock-out and fluorescent labeling，we studied Vta1, a cofactor of Vps4 to further elucidate the role of the ESCRT system in the growth of Candida albicans mycelium, Compared with Vps4, knockout of Vta1 can also affect mycelial germination and polarity maintenance, and can cause unique mycelial growth abnormalities. In addition to affecting Vps4，Vta1 can also participate in Candida albicans hyphal formation through other pathways. In addition ，we demonstrated the interaction between Vta1 and Vps4 in Candida albicans in vitro for the first time and the enhancement of Vps4 ATP hydrolase activity by Vta1. Vta1 can be used as a cofactor for Vps4 to further enhance the normal function of Vps4 in vivo, which is consistent with our study in Candida albicans in vivo.