中文题名: | 纤维素分解菌株筛选及其发酵条件的优化 |
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保密级别: | 公开 |
学科代码: | 071001 |
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学生类型: | 学士 |
学位: | 理学学士 |
学位年度: | 2014 |
学校: | 北京师范大学 |
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第一导师姓名: | |
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提交日期: | 2014-05-23 |
答辩日期: | 2014-05-16 |
外文题名: | Screening of Cellulose Decomposer and Optimization of Fermentation |
中文关键词: | |
中文摘要: |
我国有大量的落叶、秸秆等纤维素资源,将这些纤维素资源发酵形成还原糖并加工形成工业酒精,可以大大缓解我国的能源危机。通过筛选、分离合适的菌种,摸索适宜的发酵条件,可以高效地将纤维素转化为还原糖。使用脂肽类生物表面活性剂可以在不改变发酵温度、湿度等条件下大幅提高发酵效率,而且具有良好的环境相容性、无毒无害,并且可生物降解。在研究中,一共分离出了15种纤维素分解菌株,并对其中分解效率高的菌种进行了显微鉴定及脂肽最佳添加比例的研究。以分离出的高效菌株9号菌为例,经显微鉴定属于青霉属,脂肽添加最佳比例为0.02%和0.18%。发酵在进行到第10天时进入第一个平台期,第13天开始二次发酵。
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外文摘要: |
In China, there are lots of cellulose resources, such as deciduous leaf and straw. Turning these numerous cellulose resources into reducing sugar by cellulose decomposer, and then turning these reducing sugar into industrial alcohol, will easy the energy crisis in our country. By screening proper decomposer, optimizing fermentation media, the cellulose can turn into reducing sugar efficiently. The lipopeptide, a kind of bio-surfactant, will elevate the fermentation efficiency without the change of temperature, humidity, etc. And lipopeptide has good Environmental compatibility, nontoxic, and can be biodegradation. In the study, I have selected and microscopic identified 15 kinds of cellulose decomposer, and optimized the adding proportion of lipopeptide during the fermentation of the 9th strain. By microscopic identification, I know the 9th strain is one kind of penicillium, and the optimization adding proportion of lipopeptide is 0.02% and 0.18%. During the first 10 days of fermentation, the sugar yield turns into the first plateau, and the 13th day begins the secondary fermentation.
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馆藏号: | 本070401/1435 |
开放日期: | 2014-05-23 |