PET是核医学领域常用的临床检查影像技术。¹⁸F作为PET药物制备中最常用的放射性核素，具有射线能量适于成像、半衰期（T_1/2 = 109.8 min）适于较复杂合成和远距离运输等优点；使用双功能连接剂络合[¹⁸F][AlF]²⁺与肽结合是一种新型的¹⁸F标记方法。

HBED-CC（N,N'-双[2-羟基-5-(羧乙基)-苄基]乙二胺-N,N'-二乙酸）是一种性能优良的双功能连接剂，广泛应用于⁶⁸Ga，^99mTc(CO)₃，[¹⁸F]AlF等放射性核素的标记。关于⁶⁸Ga标记HBED-CC-单侧和双侧取代衍生物研究结果表明HBED-CC-双侧取代标记物具有更优越的生物学性质。目前已有[¹⁸F]AlF标记HBED-CC-单侧取代衍生物的研究，标记和生物学结果都显示出较为优良的应用前景。本研究旨在建立[¹⁸F]AlF标记HBED-CC-双侧取代衍生物的新方法，并将单侧和双侧取代标记物的理化性质与生物学性质进行对比，拓广其应用范围，弥补研究空缺，拓展HBED-CC这一双功能连接剂的应用前景。

PET is a common clinical imaging technology in nuclear medicine. ¹⁸F has a suitable position energy for imaging, and a suitable half-life (t_1/2 = 109.8 min) for radiosynthesis and long-distance transportation as a widely used radionuclide for PET imaging. Using bifunctional chelator (BFC) to chelate [¹⁸F][AlF]²⁺ with peptide is a new ¹⁸F labeling method.

HBED-CC (N,N'-bis[2-hydroxy-5-(carboxyethyl)-benzyl]ethylenediamine-N, N'-diacetic acid), a bifunctional chelator with excellent properties, is widely used in the labeling of ⁶⁸Ga，^99mTc(CO)₃，[¹⁸F]AlF and etc. The report of ⁶⁸Ga labeled monomeric and dimeric HBED-CC derivatives showed that the dimeric HBED-CC compounds had better biological properties. At present, the report of [¹⁸F]AlF labeled monomeric HBED-CC derivatives showed excellent prospect in labeling and biological properties. The purpose of this study is to design a suitable method of labeling [¹⁸F]AlF with dimeric HBED-CC derivatives, and comparing the physical chemical and biological properties between monomeric and dimeric compounds. In order to expand the application of [¹⁸F]AlF, make up for the lack of research and expand the application prospect of HBED-CC.