黑腹果蝇全基因组MicroRNA基因敲除是一种综合了反求遗传学和正向遗传筛选的优势的研究方法，且为基于表型的筛选奠定了基础。本实验初步得到了231和239两个microRNA的基因敲除果蝇。对经过染色体背景清除的microRNA敲除果蝇进行了重新平衡，利用Cre-Loxp系统去除同源重组插入的白眼基因，构建绿色荧光蛋白品系。对已确定microRNA敲除的果蝇204，143和171进行雄果蝇可育性，产卵能力和卵孵化能力的表型统计。初步确定204果蝇的雄性不育是由遗传背景造成的，143果蝇的产卵能力相较于w1118野生型果蝇无明显变化，而171果蝇具有卵孵化能力的缺陷，可能是由于胚胎发生过程受到了miRNA基因敲除的影响。

Global microRNA knock-out in Drosophila Melanogaster is a reverse genetics approach combined with advantages from forward genetics. It provides solid clue for microRNA functions via phenotype-based screening. In this experiments, several candidates are screened out and mapped for microRNA knock-out lines 231 and 239. Other microRNA knock-out candidates are rebalanced after chromosomal background cleaning. The Cre-loxp system is employed for mini-white gene removal. GFP stocks for several lines of microRNA knock-out flies were also generated. For the confirmed miRNA knock-out flies, 204, 143 and 171 lines underwent phenotypic assays including male fertility, egg-laying rate and hatching rate. Sterility of line 204 might be due to genetic background. No obvious difference is observed for egg laying rate between143 and wild type. Hatching defect of line 171 might be true phenotype caused by miRNA knock-out.