A retrospective  study  analyzed the  data  of NICU patients  in  the  Seventh Medical Center of the Chinese People's Liberation Army General Hospital between December 2014 and September 2017, and analyzed the differences and correlations of serum BG values and platelet parameters, as well as the two parameters before and after antifungal treatment. In order to explore whether platelet index combined with G test can be used for early clinical differential diagnosis of neonatal IFI. The results showed that compared with the uninfected group, the IFI group infants had significantly lower PLT, PCT and PDW values, and significantly higher BG values, MPV and P-LCR values. Compared with the bacterial group, the infants in the IFI group also had significantly lower PLT and PCT values, significantly higher BG values,  MPV  and  P-LCR  values,  and  more  complex  but  equally  significant differences in PDW values. There was a correlation between BG value and platelet parameters in IFI group. Among them, BG value was negatively correlated with PLT,  PCT  and  PDW,  and  positively  correlated  with  MPV  and  P-LCR.  After antifungal treatment, PLT, PCT and PDW increased, MPV decreased, and P-LCR did not change significantly in IFI group. Therefore, in the clinical detection of neonates, the changes of BG value and platelet parameters can be used to indicate that the patient is affected by pathogenic bacteria, and the combined detection and dynamic monitoring of the two are helpful for the early detection, diagnosis and treatment of IFI.