本文研究了以人肌酸激酶（HCK）为抗原、利用噬菌体库技术筛选出的抗体型分子伴侣scFv与人肌酸激酶的相互作用。在HCK再折叠过程中，抗体型分子伴侣scFv会与HCK中间体结合而抑制聚沉，促进其结构的恢复，但同时scFv与HCK中间体的结合也阻止了酶活性的恢复。根据前期研究结果，我们筛选出了6段可能与scFv结合的HCK寡肽片段，并探究在HCK的再折叠过程中，寡肽片段是否能够通过竞争性地与scFv结合，使HCK中间体从HCK-scFv复合体中释放出来，以促进酶结构与活性的恢复。研究结果表明，6段寡肽具有不同的竞争能力，其中一段寡肽（氨基酸序列为KGKYYPLKSMTEKEQQQL，Lys170-Leu187）与scFv具有较强的结合能力，能够使HCK的复性率提升到69%，由此推测HCK中最有可能与scFv结合的位点为Lys170-Leu187。本论文分别以蔗糖和葡聚糖70模拟拥挤环境，研究了拥挤环境中scFv-寡肽体系对HCK折叠的影响作用，发现与稀溶液相比，在蔗糖体系中，scFv-寡肽体系能够有效地促进HCK的再折叠，使其复性率达到72%。而在葡聚糖70体系中，scFv-寡肽体系的促进作用不是很明显。本论文进一步研究了scFv-寡肽体系对HCK包涵体复性的影响作用，HCK包涵体自身的稀释复性效果不佳，当包涵体复性体系中先后添加scFv与寡肽作用之后，其活力有一定程度的提升，表明scFv-寡肽体系有助于HCK包涵体的复性。

In this paper, we studied the interaction between human muscle creatine kinase (HCK) and a chaperone-like scFv antibody which is screened using HCK as antigen by phage-display method.In the refolding process of HCK, scFv could inhibit aggregation and promote recovery of HCK structure by binding to the refolding intermediat. However, the binding between scFv and HCK intermediate blocks the recovery of HCK activity. To solve this problem, we have screened six kinds of peptides which contain the same amino acid sequences as HCK segments and can be recognized by scFv. We want to know if these peptides can act as competitors of HCK for binding to scFv antibody and produce the release and reactivation of HCK from the HCK-scFv complex. The results indicate that these six kinds of peptides have different binding ability. The peptide with amino acid sequence of KGKYYPLKSMTEKEQQQL (Lys170-Leu187) has the strongest binding ability and could increase the reactivation yield of HCK to 69%. These results indicate that the most possible binding site on HCK recognized by scFv is Lys170-Leu187.We also studied the interaction between HCK and scFv in crowding environment using sucrose and dextran 70 as crowding agents. Compared with the dilute solution system, the scFv-peptide system can promote refolding and reactivation of HCK efficiently in sucrose solutions. The efficiency of the scFv-peptide system to promote refolding of HCK is low in dextran 70 solution. We further studied the effect of scFv-peptide system on the refolding of HCK inclusion bodies. The reactivation yield of HCK inclusion bodies is quite low after dilution, while the presence of scFv-peptide system could promote reactivation of HCK inclusion bodies.