哺乳动物雷帕霉素靶蛋白(mTOR)信号通路参与调控细胞生长、增殖和存活，是细胞内重要的信号途径。本文通过结构生物学的方法对 mTOR 信 号通路上游调控机制进行研究，研究对象涉及四种蛋白:胰岛素受体、EphA2、PTEN 及其突变体 PTEN C124S。实验进行到蛋白质表达阶段，四种蛋白均能够在BL21 (DE3)菌株中经 IPTG 诱导实现过表达，但目的蛋白形成包涵体。本文提出 了一种较为有效的处理包涵体的方法，从而为后续蛋白纯化与晶体筛选提供了基础。同时构建了一个重组蛋白GST-EphA2，积累了实验材料。本实验最终目的是获得复合物，研究目的蛋白间相互作用方式。

The mTOR pathway plays an important role in regulating cell growth, proliferation, and survival. This research concentrates on four target proteins which are involved in the upstream of mTOR pathway, including the insulin receptor, EphA2, PTEN and the mutant PTEN C124S. All of them can be overexpressed when induced with IPTG in BL21 (DE3). But the formation of inclusion bodies makes it difficult to carry out the following procedures, such as purification crystallization of proteins. An effective method of isolation of proteins from inclusion bodies been developed, which will be beneficial to later procedures of this research.The construction of the recombinant protein GST-EphA2 kinase domain provides another material which will be helpful in obtaining complexes. The ultimate goal of this research is to obtain complexes and to throw light on the interactions among target proteins.