利用邻氨基吡啶炔基类化合物1作为先导化合物，设计了一种新的¹⁸F标记的CSF-1受体分子探针，合成了其稳定化合物2，并采用核磁共振氢谱（¹H NMR）、氟谱(¹⁹F NMR)以及质谱（MS）对中间体和目标产物的结构进行了分析鉴定。结果表明，合成的最终产物与设计的目标化合物结构一致。测定了目标化合物2对CSF-1受体激酶活性抑制的IC₅₀值，发现化合物2的激酶活性抑制能力明显低于化合物1，说明在邻氨基吡啶炔基类化合物的苯环对位引入大的取代基会降低化合物对CSF-1受体激酶活性的抑制能力。本文结果为进一步设计性质优异的CSF-1受体分子探针提供了参考。

Using o-aminopyridine alkynyl compound 1 as lead compound, a ¹⁸F-labeled molecular probe targeting the CSF-1 receptor was designed. The reference compound (2) was synthesized. The structures of the intermediates and the target compound were analyzed and validated by ¹H NMR, ¹⁹F NMR and mass spectrometry (MS). In vitro kinase enzyme assay was performed. The results showed the decreased inhibitory activity of compound 2 against the CSF-1 receptor compared to that of compound 1, indicating introduction of large substituent at the para-position of the benzene ring decreased the inhibitory activity of the o-aminopyridine alkynyl compounds. The results are useful for further structural modifications to explore new potential radiotracers for the CSF-1 receptor.