作为细胞的能量中心，线粒体与细胞内许多基础的生物学过程紧密相关，其功能的丧失与许多疾病的发生紧密相关，例如癌症发生，代谢紊乱以及神经退行性疾病等等。线粒体的动态过程可以被粗略划分为融合，分裂，发生，自噬和凋亡，这些动态过程需要一系列的调控因子的调控。尽管有很多线粒体调控因子已经被发现，但很多线粒体相关问题尚未解决，这预示着还有很多的线粒体调节因子未被发现。为了开展线粒体调节因子的筛选研究，我建立了3000条X染色体连锁的致死品系，以此作为线粒体调节因子的筛选库。我通过果蝇中较为成熟的Flp/FRT系统的纯合突变体克隆，并选择了sqh-Mito-EYFP 作为初步的筛选工具，然后用mRpS9-GFP作为内源筛选工具作为进一步的检测。在我的研究中，我选择果蝇翅成虫盘的扁平状细胞作为我的筛选系统来观察线粒体的形态。直至现在，我已经筛选获得了9条带有不同线粒体表型的品系，有可能是潜在的新的线粒体调节因子。进而，我的内源工具也表现出与初次筛选工具同样的表型，这也加强了我对初次筛选所得结果的信心。

As the power house of the cell, mitochondria is vital for many fundamental processes, and defects in mitochondrial activity is associated with many diseases, including tumor, metabolic disorder and neurodegenerative diseases. There are many factors required in the mitochondrial dynamics, which is roughly defined as a combination of fusion, fission, biogenesis, mitophagy and apoptosis. Although many mitochondrial regulatory proteins have been identified, questions still remain, implying the potential of finding novel mitochondrial dynamics relating factors. As a resource for my screen, I established more than 3000 X chromosome-linked lethal Drosophila strains. With Flp/FRT system producing homozygous mutant clones, I ultilized sqh-Mito-EYFP as a marker for my primary screen and mRpS9-GFP as my endogenous marker for further validation. In my present study, I chose peripodial cells in the wing imaginal disc as my working model for mitochondria observation. Until now, I have found nine lines with different mitochondria phenotypes, which are potentially novel factor mutants. Furthermore, the endogenous marker shows the same phenotypes, which reinforces my confidence for my primary screen.