TPD52是在人乳腺癌细胞中发现的一种肿瘤相关抗原，具有调节囊泡运输、细胞分泌、细胞存活、增殖、迁移和DNA损伤修复等多种生物学功能，并且在很多种肿瘤细胞或者组织中都呈现出过量表达以及基因扩增的现象。人们虽然已经对TPD52基因的表达调控机制进行了研究，但这个基因的基本转录调控机制还未被完全阐明。在此研究基础上探讨TPD52转录调控的分子机制，从而有助于进一步解释在肿瘤细胞中TPD52高表达的内在机制，为进一步阐明TPD52作用的分子机制提供新线索，TPD52基因上新的调控位点的发现也将为肿瘤治疗提供新的靶点和启示。 本研究我们通过对其启动子序列进行分析，并对其上存在的潜在转录调控元件及转录因子进行研究。CREB作为其中关键的转录因子之一，是本实验的主要研究方向。

TPD52 is found in human breast cancer cell and associated with tumor antigen. TPD52 can regulate vesicle transport, cell secretion, cell survival, proliferation, migration and DNA damage repair and other biological functions, but also in a variety of tumors showed overexpression and gene amplification phenomenon. Although the regulation mechanism of TPD52 gene expression has been studied, the basic transcriptional regulation of this gene has not been fully elucidated. Based on the study of the molecular mechanism of the transcriptional regulation of TPD52, which helps to further explain the internal mechanism in tumor cells with high expression of TPD52, to provide new clues to further clarify the molecular mechanism of TPD52 function, the TPD52 gene regulatory sites for the discovery of new tumor therapy will also provide a new target and enlightenment. In this study, we analyzed the promoter sequence and studied the potential transcriptional regulatory elements and transcription factors. As one of the key transcription factors, CREB is the main research direction of this experiment.