人源乙型肝炎病毒（HBV）的感染及HBV相关疾病是全球性的公共健康问题。HBV病毒颗粒表面的包膜蛋白与肝细胞膜上的牛磺胆酸钠共转运多肽（NTCP）相结合，可介导病毒颗粒进入细胞。然而目前有许多证据表明，HBV感染还需要其他宿主的细胞因子的参与。本文以NTCP相互作用蛋白A1为研究对象，通过对分泌的抗原水平及细胞内RNA水平的检测，发现沉默基因A1可以有效地降低HBV的感染。而在HBV感染建立后对A1进行沉默，对HBV的感染并未造成影响。这表明A1可能影响HBV的早期感染阶段而非复制及转录阶段。另外，伴侣蛋白B1对A1的运输及定位具有重要的作用，内源过表达具有功能性的A1需要等量B1的辅助。

Human hepatitis B virus (HBV) infection and HBV-related diseases remain a major public health problem. Cellular entry of HBV virus is mediated by the interaction between HBV envelope proteins and sodium taurocholate cotransporting polypeptide (NTCP) on hepatocyte membrane. However, there is a lot of evidence indicating that the infection of HBV requires other factors in host cells. In this study, we chose NTCP interacting protein A1 as research object, and found that silencing A1 can efficiently reduce HBV infection, as indicated by secreted antigen and intracellular RNA amount. If silencing A1 occur post HBV infection establishment, it seems that HBV infection is not compromised. It suggests that A1 probably affect HBV early infection but not replication or transcription process. Because partner protein B1 is responsible for A1 trafficking and localization, exogenous expression of functional A1 requires equal amount of B1.