中文题名: | 葡萄糖氧化酶在NH2+注入ITO电极上电化学行为的研究 |
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保密级别: | 公开 |
学科代码: | 070301 |
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学生类型: | 学士 |
学位: | 理学学士 |
学位年度: | 2009 |
学校: | 北京师范大学 |
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提交日期: | 2009-06-18 |
答辩日期: | 2009-05-20 |
中文关键词: | |
中文摘要: |
用离子注入技术,将NH2+注入ITO表面,制成NH2/ITO电极。结合纳米技术,制成AuNPs/NH2/ITO电极。用红外光谱、原子力显微镜、扫描电子显微镜和电化学阻抗研究电极的表面形态和特征。结果表明NH2+注入了ITO表面,使得表面粗糙度增加,表面积增大,阻抗增大;纳米金修饰电极比NH2/ITO电极的阻抗小,电子转移容易,且对葡萄糖氧化酶的吸附能力增强。将葡萄糖氧化酶(GOD)修饰该电极上制成GOD/AuNPs/NH2/ITO电极,用循环伏安法对GOD在AuNPs/NH2/ITO电极的电化学行为进行研究并测定相关参数。实验表明,电极过程是受吸附控制的准可逆过程,纳米金促进了GOD的还原。根据Laviron理论,求得电极反应速率常数ks=3.37 s-1,电荷转移系数α=0.52。
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外文摘要: |
The NH2+ was implanted into the ITO films to prepare the NH2/ITO electrode. Combined with nanometer technique, the AuNPs/NH2/ITO electrode was prepared. We used infrared spectroscopy, atomic force microscope and scanning electron microscopy and electrochemical impedance to study the electrode surface morphology and characteristics. The results showed that the NH2+ had been put into the ITO surface and the surface roughness and surface area increased. What’s more, the impedance increased, too. The impedance of the electrode modified with nanometer gold in which the electrons transferred more easily was much lower. Moreover, its capacity to absorb glucose oxidase was enhanced. The AuNPs/NH2/ITO electrode was modified with the glucose oxidase (GOD). By the cyclic voltammetry, we studied the electrochemical behavior and determined the relevant parameters. The experimental results show that the electrode process is controlled by the quasi-reversible adsorption process, and nanometer gold promoted the reduction of GOD. According to the Laviron theory ,we obtained the electrode reaction rate constant ks=3.37 s-1, and the charge transfer coefficient α = 0.52.
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插图总数: | 0 |
插表总数: | 0 |
馆藏号: | 本070301/0949 |
开放日期: | 2009-06-18 |