神经胶质瘤是源自神经胶质细胞或前体细胞的肿瘤。它是原发性脑组织和其他中枢神经肿瘤类型中最常见的恶性肿瘤。神经胶质瘤可以基于EM/PM分类系统被分为三种亚型。课题组前期工作发现在PM亚型胶质瘤的生长过程中，在前髓鞘形成少突胶质细胞（Pre-myelinating oligodendrocyte，Pre-MO）分化至髓鞘形成少突胶质细胞（Myelinating oligodendrocyte，MO）的阶段出现分化阻滞，并同时出现增殖频率不高的现象。我们从肿瘤形成的微环境出发，以PM亚型胶质瘤对神经元轴突有依赖作用为假设，初步探究神经元轴突对PM亚型胶质瘤形成的作用。

在本次研究中，我们将搜集到的神经元基因和突触基因通过Qlucore、R语言等生信软件，把它们在EM/PM亚型胶质瘤细胞中的差异表达情况进行对比分析，发现PM亚型胶质瘤细胞中高表达一些神经元基因和突触基因，并且通过GO富集分析对这些基因所参与的生物学功能进行初步的了解。GO富集结果显示这些高表达的神经元基因和突触基因均参与了化学突触释放和后续通过离子通道交流等过程，且基因的位置大都在突触后膜上。这些结果共同表明，神经元轴突与PM亚型神经胶质瘤之间可能存在多种机制的交流可以提供增殖频率不高的胶质瘤以支持，影响PM亚型胶质瘤的生存发展。

Gliomas are tumors that originate from glial or progenitor cells. It is the most common malignant tumor among primary brain tissue and other types of central nervous system tumors. Based on the EM/PM classification scheme, gliomas could be classified into three subtypes. According to the accumulation of work before, there exists a blockage during the differentiation stage of pre-myelinating oligodendrocyte(Pre-MO) to myelinating oligodendrocyte(MO) while the proliferation frequency was not high in the process of PM subtype glioma growth. Beginning with microenvironment of gliomas, we preliminarily explored the effect of neuronal axons on the formation of PM subtype glioma, assuming that PM subtype glioma is dependent on neuronal axons.

We used Qlucore, R language and other bioinformatic software to compare the differential expressions of neuronal genes and synaptic genes in EM/PM subtype glioma cells. We found that some neuronal genes and synaptic genes were highly expressed in PM subtype glioma cells. GO enrichment results showed that these neuronal genes and synaptic genes,  which expressed highly on PM gliomas, were involved in chemical synaptic release and subsequent communications through ion channels, and most of the genes were located on the postsynaptic membrane. Together, these results suggest that there may be multiple mechanisms of communication between neuronal axons and PM subtype gliomas, which provide support for gliomas with low proliferative frequency and affect the survival and development of PM subtype gliomas.