脑功能是人类生命活动的基础，活体脑内生物化学分子的定量和定性分析对于探究脑功能与疾病的机制具有重要意义。多巴胺（dopamine，DA）是人脑内的重要神经递质，参与调控行为、学习记忆、情绪等关键神经功能。近年研究发现维生素C（vitamin C，V_c）能够作为神经调质，可能参与调节帕金森等DA相关的神经生理病理过程。在活体水平探究脑内V_c与DA水平的关系，能够为理解DA相关的神经过程机制、探索V_c的神经功能提供关键实验支持。然而，脑内具有复杂的分子、化学环境，DA与V_c都具有不稳定的特性，且DA与V_c在电化学检测中相互干扰，实现V_c作用下DA的定量分析，对分析方法提出挑战。本研究利用快速扫描循环伏安法结合深度学习算法，通过体外单一组分和混合组分的分析，实现了存在V_c浓度变化干扰条件下DA的精准定量分析，并实现了腹腔注射V_c后鼠脑纹状体DA的释放量变化的定量分析。为研究多种病理生理过程中V_c与DA的作用及机制提供重要实验基础。

Brain functions as the basis of human vital  activities.  Quantitative analysis of neurochemical molecules in living brain is essential not only for understanding the mechanism of brain function but also for developing potential treatment for neurological diseases. Dopamine (DA),one of the prominent neurotransmitters， is fundamental for maintaining proper functions in brain，including behavior， learning and memory，and emotions. Vitamin C(V_c) is   a versatile  neuromodulator， regulating various of dopaminergic functions and disorders. Thus，studying the interaction of the intracerebral level of V_c and DA in vivo would lay the basis for further understanding the mechanism of dopaminergic system and the neural function of vitamin C. However，quantitative analysis of DA and V_c in vivo is challenged by the complex molecular and chemical environment in brain. The unstable nature of DA and V_c，as well as their close oxidation potentials，further complicate simultaneous analysis of these chemicals，which poses a challenge to the analysis method. In this study，taking advantage of recently developed fast-scan cyclic voltammetry combined with deep learning-based voltammetric，we obtained the linear relationship between concentration-current response of DA in the presence of V_c，and analyzed single components and mixed components in vitro. 

We further carried on quantitative analysis of the change of DA release in the rat striatum after intraperitoneal injection of V_c. Our study provides the experimental basis for further studying the role and mechanism of V_c in various dopaminergic pathophysiological processes.