人体中每时每刻都在发生着成千上万的化学反应，反应物和产物的运输机制对于维持人体正常的新陈代谢功能非常重要。因为细胞中有许多分子不能直接穿过细胞膜，所以必须借助其他载体来实现这些分子的跨膜运输，囊泡运输就是跨膜运输的重要途径之一。而Sys1蛋白是一种与囊泡运输调节有关的膜蛋白，在囊泡运输调控机制中发挥着非常重要的作用。以前的研究表明在酵母菌细胞中，Sys1蛋白的缺失将导致高尔基体膜不能与一些与囊泡运输有关的调控器，如Arl3p、Arl1p和GRIP结构域等特异性结合，导致囊泡运输受阻。因此，有研究者得出假设：Sys1蛋白可能作为Arl3p的接收器参与到囊泡运输调节中。但是目前对于Sys1蛋白的研究少之又少，本毕业设计旨在通过优化Sys1蛋白晶体生长条件，获得纯度较高且聚集态单一的Sys1蛋白的晶体，并解析其晶体结构，以进一步研究囊泡运输相关机制。

There are thousands of chemical reactions occur in one’s body at all times, so the transport mechanism of reactants and products is very important to maintain the normal metabolic function of one’s body. It is necessary for cells to use some carriers to support transmembrane transport of those molecules which cannot pass through the cell membrane directly only depending on themselves. Vesicle transport is one of the most important ways to achieve transmembrane transport. Sys1 which is an integral membrane protein involved in the regulation of vesicle transport plays an important role in the regulation mechanism of vesicle transport. Previous studies have shown that in yeast cells, the loss of Sys1 would result in that the Golgi membrane cannot specifically bind to the Arl3p, Arl1p and GRIP domains, which are regulators involved in vesicle trafficking. Therefore, some researchers have hypothesized that Sys1 may be used as a receiver for Arl3p in vesicular transport regulation. However, there are few studies on Sys1. My graduation study design aims to obtain crystals of Sys1 with high purity and aggregation state by optimizing the crystal growth conditions of Sys1, and to analyze its crystal structure. The analysis of Sys1 protein structure will promote the development of vesicle transport.