本论文首先在综述部分阐述了植物耐盐性机制与SOS(salt overly sensitive) 基因家族的关系。其次在实验部分，为了更好地研究植物耐盐通路中的SOS2激酶的上游磷酸酶，通过分子生物学手段，例如PCR，酶切，连接等实验方法，构建目的基因表达载体，并用电转法构建带有表达载体的大肠杆菌克隆和农杆菌克隆，最后利用农杆菌转染拟南芥，并根据抗性筛选转基因植株。最终，成功构建了10个PP2C类磷酸酶的克隆；获得了3种碱性磷酸酶过表达的转基因植株。为未来研究SOS2上游磷酸酶奠定了实验基础。

To explore the up-stream phosphatase of SOS2, we constructed transgenic arabidopsis, in which the phosphatases belonging to PP2C can overly express. The vector containing target gene can be constructed via PCR, enzyme cutting, and linking with ligase. Then, we transfer the vector into Ecoli and agrobacterium to make the clones，and the transfection of arabidopsis will be completed with the help of agrobacterium. Finally, the target seeds of transgenic arabidopsis can be selected in the Hc LB plate. Finally, 10 clones of different genes are precisely made, and 3 kinds of transgenic arabidopsis were successfully obtained.